2.5×SYBR Green One Step RT-PCR
Cat.No：SR1112 Solarbio
Storage：-20℃, avoid light.
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SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
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TGF-β/Smad
Hippo
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Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
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Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Storage | -20℃, avoid light. |
| Unit | kit |
| Specification | 50T 200T |
This product is a one-step Real-Time RT-PCR reagent. The included SYBR Green I fluorescent dye binds to all double-stranded DNA, allowing the product to be used to detect a wide range of different target sequences without synthesizing specific labeled probes. When using this product, reverse transcription and quantitative PCR are performed in the same reaction without adding reagents or openning the cap, avoiding contamination and improving experimental efficiency. Loss of RNase H activity in a new and efficient reverse transcriptase reduces RNA degradation in reverse transcription reactions. The enzyme has a high reverse transcription efficiency and can perform a good reverse transcription reaction on a small amount of RNA template. RNA templates with high affinity for RNA, high GC content, and complex secondary structure. The new high-efficiency hot-start enzyme is actively blocked at room temperature, thus avoiding non-specific amplification due to non-specific binding of primers and templates or primer dimers, improving the accuracy of real-time PCR reactions. The included buffer system maximizes the efficiency of both enzymes. This product has high sensitivity, high specificity, and a wide linear range, which makes the quantification of the target gene more accurate.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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