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My Cart> Cell Biology > Cell Detection > Cell Growth Factor > Recombinant Human HMGB1/HMG-1Purity：≥95% as determined by reducing SDS-PAGE.
Storage：Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Appearance：Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0.
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| Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0. |
| Purity | ≥95% as determined by reducing SDS-PAGE. |
| Storage | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| Transport Condition | The product is shipped at ambient temperature. |
| Background | High mobility group protein B1 is a member of the HMGB family consisting of three members，HMGB1，HMGB2 and HMGB3.It Contains 2 HMG box DNA-binding domains entitled box A and box B and It is a highly negative-charged C terminus.As a nuclear protein，HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival.Meanwhile，it is revealed that HMGB1 can also act as a cytokine extracellularlly and regulates monocyte，T cell，dendritic cell activities in inflammatory responses. |
| Source | Human cells |
| Solvent | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Endotoxin | Less than 0.1 ng/ug(1 IEU/ug)as determined by LAL test. |
| Unit | Piece |
| Specification | 10ug 50ug 500ug 1mg |
Recombinant Human High Mobility Group Protein B1 is produced by our Mammalian expression system and the target gene encoding Gly2-Glu215 is expressed with a 6His tag at the C-terminus.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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