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My Cart> Cell Biology > Cell Detection > Cell Growth Factor > Recombinant Human CD40 Ligand/TNFSF5Purity：≥95% as determined by reducing SDS-PAGE.
Storage：Powder:＜-20℃ for long store;RT，3 weeks.Liquid:4-7℃，2-7 days;＜-20℃，3 months.
Appearance：Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0.
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| Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0. |
| Purity | ≥95% as determined by reducing SDS-PAGE. |
| Storage | Powder:＜-20℃ for long store;RT，3 weeks.Liquid:4-7℃，2-7 days;＜-20℃，3 months. |
| Transport Condition | The product is shipped at ambient temperature. |
| Background | CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily.Like other TNF superfamily members，CD40LG exists as a trimer in membrane bound and soluble form，both of which are bioactive.CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells.CD40LG is a differentiation antigen that is expressed on the surface of T-cells.It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines.CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes.It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma，TNF-alpha，and IL2. |
| Source | E.coli |
| Solvent | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Endotoxin | Less than 0.1 ng/ug(1 IEU/ug)as determined by LAL test. |
| Unit | Piece |
| Specification | 10ug 50ug 500ug 1mg |
Recombinant Human CD40 Ligand is produced by our E.coli expression system and the target gene encoding Met113-Leu261 is expressed.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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