Dialysis Membranes MWCO 3.5kD
Cat.No：YA1077 Solarbio
Storage：RT
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> Instruments & Consumables > Basic Consumables > Filter & Change & Concentrate Consumables > Dialysis Membranes MWCO 3.5kD
Product Information
| Name | Dialysis Membranes MWCO 3.5kD |
| Material | Regenerated cellulose (RC) membranes |
| Storage | RT |
| Instruction | Flat width 34mm. MWCO 3.5kD. |
| Unit | Roll |
| Specification | 5M |
Common dry dialysis membranes (American combined carbated, glycerol coated, treated before use)
Technical Parameters
1. PH stability range: 5-9.
2. Pollutant level: sulfide < 0.3%; Heavy metal<50ppm.
3. Chemical compatibility: Compatible with many salts such as CaCL2, (NH4)2SO4 or aqueous and organic solvents commonly used in molecular biology and enzymology, such as isopropanol, ethanol and acetone.
4. Temperature resistance: It can be boiled and autoclave.
5. Potein adsorption: The adsorbed protein content per gram of dialysis membranes was less than 1ng.
Pre-processing
1. Cut the Dialysis membranes into an appropriate length of 10-20cm.
2. Boil the Dialysis membranes for 10 minutes in 2% (w / v) NaHCO3 and 1mmol/L EDTA (pH 8.0) solution.
3. Wash the Dialysis membranes thoroughly with distilled water.
4. Boil the Dialysis membranes for 10 minutes in 1mmol/L EDTA (pH 8.0) solution.
5. After cooling, store at 4 ℃ and ensure that the Dialysis membranes is always immersed in solution. From this step, the later operation needs to wear gloves.
6. Clean the interior Dialysis membranes with distilled water before use.
*The experimental requirements are not strict can be used in the following simple treatment method: Boil in boiling water for 10 min and ready for use.
Application
Remove salts, surfactants, and solvents
Buffer displacement and PH adjustment of the sample solution
Concentrate the proteins, polypeptides, and antibodies
DNA electroelution
Preparation of diluted proteins before electrophoresis
Elimination of small-molecule pollutants
Extracted and purified of tissue culture
Combined research
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Note: The material of dry dialysis bag is RC (regenerated cellulose); The material of the ready-to-use dialysis bag is CE (biotechnology grade cellulose ester membrane) or RC (regenerated cellulose). For specific product materials, please refer to the official website instructions.
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我们需要用透析袋截流10kd的蛋白，请问何种分子孔径的透析袋可否使用? 2022-07-14 09:32:06
Administrator建议选择截留分子量为小于等于目的蛋白分子量二分之一的透析袋