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Storage：RT，3 years
Application： Used for the cultivation of nitrogen-fixing bacteria
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| Name | Nitrogen-Free Agar Medium |
| Storage | RT，3 years |
| Application | Used for the cultivation of nitrogen-fixing bacteria |
| Unit | Bottle |
| Specification | 250g |
Media formulation (g/L): Mannitol 10.0g/L; K2HPO 40.2g/L; MgSO4·7H2O 0.2g/L; NaCl 0.2g/L; CaCO3 5.0g/L; Agar 15.0g/L; pH7.2±0.2 (25℃);
Preparation Method: Dissolve30.6gto1000mLdistilledwater, heatand continue to boil for more than 1 minute, divide while stirring, subpackingand115℃highpressuresterilizationfor30minutestouse.Useforantibioticsensitivitytest.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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