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My Cart> Molecular Biology > PCR/qPCR > Nucleic Acid Amplification Enzyme & Premix > Quickstart Hot Start Enzyme Premix
Storage：Storage at 4℃ for short time,? -20℃?for longer time.
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| Storage | Storage at 4℃ for short time,? -20℃?for longer time. |
| Note | 1. Quickstart Premix plus (Probe qPCR) uses a new hot start enzyme, which can achieve rapid hot start in 1 to 5 minutes; After special preparation of Buffer system, it is suitable for multiple fluorescence quantitative PCR reaction. 2. With higher specificity, it can significantly improve the sensitivity of fluorescence quantitative PCR limit detection, and significantly improve the normalization of amplification curve and fluorescence value of the very low concentration template, which is suitable for use as a high-sensitivity fluorescence quantitative PCR detection reagent. 3. The three-step method is recommended for primers with low annealing temperature or for amplification of long fragments over 200 bp. 4. Before and after amplification, please use special areas and pipettes, wear gloves to operate and change frequently; Do not open the reaction tube after the PCR reaction is completed to minimize the contamination of the PCR products to the sample. |
| Unit | Branch |
| Specification | 100T |
Quickstart Hot Start Enzyme Premix is a special reagent for qualitative and quantitative reaction of Real Time PCR using probe method. The new hot-start enzyme Quickstart Taq plus, which contains Taq enzyme activity blocked at room temperature, can effectively inhibit non-specific amplification caused by primer non-specific annealing or primer polymers at low temperatures, improve the specificity of amplification reaction, and obtain more stable detection results for very low concentration templates. This reagent uses a special Buffer for qPCR with optimized formulation, which can obtain a good standard curve in a wide quantitative region and accurately quantify. This reagent is compatible with fluorescent quantitative PCR instruments from many manufacturers, such as AppliedBiosystems, Eppendorf, Bio-Rad and Roche.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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