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RNase R

Cat.No：R8580 Solarbio

Storage：-20℃

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Product Information

Storage	-20℃
Background	Reaction buffer: 200mM Tris-HCl (pH 8.0 at 25℃), 1M KCl, 1mM MgCl2
Note	1. The exertion of RNaseR activity requires 0.1-1.0mM Mg2+.2. With the increase of substrate RNA, the digestion time and enzyme amount can be appropriately prolonged.3. The EDTA content in RNA samples may affect the activity of RNaseR.4. circRNA or lasso structure RNA may decrease in abundance after prolonged RNaseR digestion, maybe it's digestive ability of tolerate is weak, please reduce the amount of RNaseR or digestion time.
Source	Recombinant E.coli
Unit Definition	Under the condition of 37 ℃ standard reaction, the amount of enzyme required to convert 1μg poly(A) to acid-soluble nucleotides for 10min is defined as 1U.
Concentration	20U/ul
Solvent	50mMTris-HCl(pH7.5at25℃),100mMNaCl,0.1mMEDTA,1mMDTT,0.1%TritonX-100(v/v),50%Glycerol(v/v)
Unit	Set
Specification	50U 200U 300U 1KU

RNase R is a Mg2+ dependent exonuclease derived from the E.coli RNR superfamily that completely hydrolyzes linear RNA into dinucleotides and trinucleotides in the 3'→5' direction. It is not sensitive to circular RNA or double-stranded RNA, and can be used for the production of special structural RNA, such as circRNA, lariatRNA, double-stranded RNA with 3' protruding end less than 7 nucleotides, and tRNA with complex structure. RNaseR is commonly used in gene expression and variable shear studies, and can digest linear RNA to enrich circRNA or lariatRNA.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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