Red fluorescent nucleic acid dye

Red fluorescent nucleic acid dye is a highly sensitive fluorescent dye, which is often used for nucleic acid staining after electrophoresis of agarose and polyacrylamide gels. After combining with DNA, the dye emits orange-red fluorescence under the excitation of ultraviolet light transmisometer, which can be photographed by Polaroid film or gel imaging processing system with CCD imaging head. Complexes that bind DNA dyes have fluorescence yields 20-30 times higher than dyes that do not bind DNA, so bands as small as 10ng of DNA can be detected when free red fluorescent nucleic acid dyes (0.5μg/mL) are present in the gel.

How to use:

1. Glue dyeing: Melt 100mL agar-agar gel solution (concentration is generally 0.8-2%) in the microwave oven, cool to about 60℃ (when not hot), add 5-10μL dye, shake gently and pour glue (avoid bubbles), after the glue is completely solidified, sample electrophoresis, after electrophoresis, observe and take photos under the purple lamp.

(Note: The electrophoretic mobility of linear DNA is reduced by about 15% in the presence of the dye).

2. Foam dyeing: After agarose gel electrophoresis, the gel is immersed in electrophoretic buffer containing dye (3μg/mL) or deionized water, and stained at room temperature for 15-45min (depending on gel thickness). When decolorizing, soak in deionized water or 1mM MgSO4 solution at room temperature for about 10-30min to reduce the background fluorescence (optional).

Note:

1, This product is a strong mutagen, please pay attention to safety when using.

2. For your safety and health, please wear a lab coat and disposable gloves.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.