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My Cart> Molecular Biology > Recombinant Clone Expression > Competent Cell > GT115 Competent cells
Storage：Store at -70℃
Appearance：Dry ice transport, only 10kg dry ice fee
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| Name | GT115 Competent cells |
| Appearance | Dry ice transport, only 10kg dry ice fee |
| Storage | Store at -70℃ |
| Prokaryotic Resistance | Sensitive to Amp，Kan，Spe，Chl and Tet. Resistant to Str. |
| Product Description | The genome of strain GT115 contains pir gene, which can express π protein, which is used to recognize R6K replication initiator. Plasmids carrying R6K replication initiators (such as pCpGL-Basic, pCpGL-CMV/EF1, etc.) can be replicated and amplified in this strain. By deleting sbcC and sbcD genes in the genome, the SbcCD complex of recognizing and cutting hairpin structure could not be formed in GT115 cells, and the stability of plasmid containing hairpin structure DNA was enhanced in cells. This strain contains the gene rpsL (StrA) and is resistant to streptomycin. This strain has nuclease (endA) mutation and recombinase (recA) mutation, which enhance the stability of foreign DNA. GT115 receptor cells were prepared by special technology, and the conversion efficiency of pUC19 was greater than 1×108CFU/μgDNA. |
| Note | 1，the receptive cells should be stored at -70℃，not repeatedly frozen and thawed and placed for too long，in order to avoid reducing the conversion efficiency of the receptive cells. 2，the conversion operation should be carried out according to the requirements of the corresponding temperature and aseptic conditions. 3. In order to prevent the conversion experiment from being successful，part of the connected reaction liquid can be retained to re-convert and minimize the loss. |
| Unit | Bag |
| Specification | 20*100ul |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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