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My Cart> Molecular Biology > Recombinant Clone Expression > Competent Cell > HT115(DE3) Competent cells
Storage：Store at -70℃
Appearance：Dry ice transport, only 10kg dry ice fee
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| Name | HT115(DE3) Competent cells |
| Appearance | Dry ice transport, only 10kg dry ice fee |
| Storage | Store at -70℃ |
| Prokaryotic Resistance | Sensitive to Amp，Kan，Spe，Chl and Str.Resistant to Tet . |
| Competence | F-mcrAmcrB，IN(rrnD-rrnE)1 rnc14::Tn10λ(DE3 lavUV5::T7 polymerase) |
| Product Description | HT115(DE3) is a RNASEIII-deficient Escherichia coli strain, which can be expressed by IPTG induction to produce dsRNA. For RNAi interference test. This strain also has the DE3 region of λ phage, which can express T7RNA polymerase, and is suitable for efficient expression of prokaryotic expression vectors containing T7 promoters (such as pET). Non-t7 promoter expression vectors (such as pGEX, pMal, pTrc, etc.) can also be expressed in this strain. HT115(DE3) receptor cells were prepared by a special process, and the conversion efficiency of pUC19 plasmid was 1×106CFU/μgDNA. |
| Note | 1，the receptive cells should be stored at -70℃，not repeatedly frozen and thawed and placed for too long，in order to avoid reducing the conversion efficiency of the receptive cells. 2，the conversion operation should be carried out according to the requirements of the corresponding temperature and aseptic conditions. 3. In order to prevent the conversion experiment from being successful，part of the connected reaction liquid can be retained to re-convert and minimize the loss. |
| Unit | Bag |
| Specification | 20*100ul |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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