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My Cart> Molecular Biology > Recombinant Clone Expression > Competent Cell > BL21-CodonPlus (DE3)-RIPL Competent cells
Storage：Store at -70℃
Appearance：Dry ice transport, only 10kg dry ice fee
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| Name | BL21-CodonPlus (DE3)-RIPL Competent cells |
| Appearance | Dry ice transport, only 10kg dry ice fee |
| Storage | Store at -70℃ |
| Prokaryotic Resistance | Resistant to Tet，Chl，Str，and Spe. Sensitive to Amp and Kan. |
| Competence | F–ompT hsdS(rB–mB–) dcm+ TetR gal λ(DE3) endA Hte [argU proL CamR] [argU ileY leuWStrep/SpecR] |
| Product Description | BL21-CodonPlus(DE3)-RIPL belongs to the Bacillus macroenteri B strain. Lon egg white enzyme and OmpT protease deficient type. The BL21-CodonPlus(DE3)-RIPL strain can provide four additional amino acids corresponding to the rare tRNA argU (AGA, AGG), ileY(AUA), proL (CCC), leuW (CUA). The recombinant plasmid constructed based on pACYC vector can provide additional copies of argU and proL-tRNA genes. The recombinant plasmid based on pSC101 expressed additional copies of argU, ileY and leuW-tRNA genes. Improve the expression of foreign genes rich in AT or GC sequences in E. coli. This strain also has the DE3 region of λ phage, which can express T7 RNA polymerase, and is suitable for efficient expression of prokaryotic expression vectors (such as pET) containing T7 promoters. Non-t7 promoter expression vectors (such as pGEX, pMal, pTrc, etc.) can also be expressed in this strain. The receptor cells were made by a special process, and the conversion efficiency of pUC19 plasmid was 1×106CFU/μg DNA. |
| Note | 1，the receptive cells should be stored at -70℃，not repeatedly frozen and thawed and placed for too long，in order to avoid reducing the conversion efficiency of the receptive cells. 2，the conversion operation should be carried out according to the requirements of the corresponding temperature and aseptic conditions. 3. In order to prevent the conversion experiment from being successful，part of the connected reaction liquid can be retained to re-convert and minimize the loss. |
| Unit | Bag |
| Specification | 20*100ul |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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