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My Cart> Molecular Biology > Recombinant Clone Expression > Competent Cell > ClearColi BL21(DE3) Competent Cells
Storage：Store at -70℃
Appearance：Dry ice transport, only 10kg dry ice fee
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| Name | ClearColi BL21(DE3) Competent Cells |
| Appearance | Dry ice transport, only 10kg dry ice fee |
| Storage | Store at -70℃ |
| Prokaryotic Resistance | Sensitive to Amp，Kan，Spe，Chl，Str and Tet |
| Competence | F- ompT hsdSB (rB - mB -) gal dcm lon λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) msbA148 gutQ kdsD lpxL lpxM pagP lpxP eptA |
| Product Description | lipopolysaccharide (LPS) is a component of the outer membrane of almost all gram-negative bacteria. LPS is recognized by TLR4/MD-2 on mammalian immune cells and thus activates the NF-kappB signaling pathway, leading to the occurrence of the organism's inflammatory response. The residual LPS in E. coli genetically engineered products can greatly affect downstream experiments. The outer membrane of ClearColi is the modified Lipid IVA. Compared with wild-type LPS, the modified lipid IVA lacks oligosaccharides and two secondary acyl chains. Lipid IVA does not induce the formation of activated hTLR4/MD-2 complex. Endotoxin reaction in the LPS-TLR4/NF-kB signaling pathway is avoided. In addition, lipid IVA, which does not contain oligosaccharides, is more easily removed from recombinant proteins. The growth rate of ClearColi BL21 (DE3) cells was about 50% that of normal BL21 (DE3) cells. The transformed colony appears very small for the first 24 hours. It is suggested that the transformed plates should be cultured in a culture dish for 36-48 hours and then selected for the experiment. Before induction, a longer growth time is required to achieve the desired cell density. Recombinant protein for expression of low endotoxin. This strain contains T7 RNA polymerase and E. coli RNA polymerase, which is suitable for the efficient expression of prokaryotic expression vectors containing T7 promoters (such as pET, etc.) and non-T7 promoter expression vectors (such as pGEX, pMal, pTrc, etc.) in the prokaryotic system |
| Note | 1，the receptive cells should be stored at -70℃，not repeatedly frozen and thawed and placed for too long，in order to avoid reducing the conversion efficiency of the receptive cells. 2，the conversion operation should be carried out according to the requirements of the corresponding temperature and aseptic conditions. 3. In order to prevent the conversion experiment from being successful，part of the connected reaction liquid can be retained to re-convert and minimize the loss. |
| Unit | Bag |
| Specification | 10*100ul |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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