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Application： ELISA
Reactivity：Human
Sensitivity：6.25 pg/ml
Storage：2-8℃，6 months
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| Name | Human IkB alpha/NFKBIA ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | IkB alpha/NFKBIA |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 12.5-800 pg/ml |
| Sensitivity | 6.25 pg/ml |
| Specificity | Do not react with Human NFKBIA analogue etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P25963 |
| Gene ID | 4792 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人NFKBIA单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人NFKBIA会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗人NFKBIA抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人NFKBIA发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人NFKBIA，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人NFKBIA浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人NFKBIA的浓度。 |
| Background | Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor，alpha (IkB alpha，NFKBIA，or IKBA)，is a member of the NF-kappa-B inhibitor family that function to inhibit the NF-kB transcription factor. NFKBIA inhibits NF-kB by masking the nuclear localization signals (NLS) of NF-kB proteins and keeping them sequestered in an inactive state in the cytoplasm. Also，NFKBIA blocks the ability of NF-κB transcription factors to bind to DNA，which is required for NF-kB's proper functioning. Signal-induced degradation of I kappa B alpha exposes the nuclear localization signal of NF-kappa B，thus allowing it to translocate into the nucleus and activate transcription from responsive genes. An autoregulatory loop is established when NF-kappa B induces expression of the I kappa B alpha gene and newly synthesized I kappa B alpha accumulates in the nucleus where it negatively regulates NF-kappa B-dependent transcription. As part of this post-induction repression，the nuclear export signal on I kappa B alpha mediates the transport of NF-kappa B-I kappa B alpha complexes from the nucleus to the cytoplasm. Deletion of NFKBIA has an effect that is similar to the effect of EGFR amplification in the pathogenesis of glioblastoma and is associated with comparatively short survival. Polymorphisms in NFKBIA may be important in pre-disposition to and outcome after treatment，of multiple myeloma (MM). The NFKBIA gene product，IkappaBalpha，binds to NF-kappaB preventing its activation and is important in mediating resistance to apoptosis in B-cell lymphoproliferative diseases. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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