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Application： ELISA
Reactivity：Human
Sensitivity：125 pg/ml
Storage：2-8℃，6 months
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| Name | Human DPP2 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | DPP2 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 250-16000 pg/ml |
| Sensitivity | 125 pg/ml |
| Specificity | Do not react with Human DPP2 analogue. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | Q9UHL4 |
| Gene ID | 29952 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人DPP2单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人DPP2会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗人DPP2抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人DPP2发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人DPP2，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人DPP2浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人DPP2的浓度。 |
| Background | DPP7 (dipeptidylpeptidase 7)，also known as DPPII and DPP2，is a post-proline cleaving aminopeptidase expressed in quiescent lymphocytes. Dipeptidyl peptidases (DPPs) have post-proline dipeptidyl aminopeptidase activity，cleaving Xaa-Pro dipeptides from the N-termini of proteins. DPPs mediate regulatory activity of their substrates and have been linked to a variety of diseases including type 2 diabetes，obesity and cancer. DPPs can bind specific voltage-gated potassium channels and alter their expression and biophysical properties and may also influence T cells. DPP proteins include DPRP1，DPRP2，DPP3，DPP7，DPP10，DPPX and CD26. It localizes to lysosomes. DPP7 localizes to lysosomes and exists as a homodimer via its leucine zipper motif and is involved in the degradation of oligopeptides. In response to calcium release，it can be secreted in its active form. It is essential for lymphocyte survival，as the inhibition of DPP7 results in quiescent cell apoptosis. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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