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My Cart> Molecular Biology > Recombinant Clone Expression > Competent Cell > NEB10-beta Electrocompetent cells
Storage：Store at -70℃
Appearance：Dry ice transport, only 10kg dry ice fee
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| Name | NEB10-beta Electrocompetent cells |
| Appearance | Dry ice transport, only 10kg dry ice fee |
| Storage | Store at -70℃ |
| Prokaryotic Resistance | Sensitive to Amp，Kan.Resistant to Str. |
| Competence | araD139△(ara，leu)7697 fhuA lacX74 galK16 galE15 mcrA80d(lacZ M15)recA1 relA1 endA1 nupG rpsL rph spoT1△(mrrhsdRMS-mcrBC) |
| Product Description | NEB 10-beta electroreceptor cells can only be used for electric shock transformation, not heat shock transformation. This strain is a DH10B derivative strain of Escherichia coli K12, which is especially suitable for library construction and cloning or amplification of large plasmid skeleton such as BAC and Cosmid. The characteristics of DNA recombination defect (recA1) and endA1 defect (endA1) are conducive to the stability of inserted DNA and the extraction of high purity plasmid DNA. This strain has the characteristics of anti-T1 phage infection, and can also be used in blue/white spot screening experiments to detect β-galactosidase activity, without adding IPTG, only adding X-gal can be used. The conversion efficiency of pUC19 plasmid was greater than 1010CFU/μg. |
| Unit | Bag |
| Specification | 20*50ul |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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