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My Cart> Molecular Biology > Recombinant Clone Expression > Competent Cell > AGL1(pSoup) Chemically Competent Cell
Storage：Store at -70℃
Appearance：Dry ice transport, only 10kg dry ice fee
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| Name | AGL1(pSoup) Chemically Competent Cell |
| Appearance | Dry ice transport, only 10kg dry ice fee |
| Storage | Store at -70℃ |
| Competence | C58 RecA(rifR/carbR) Ti pTiBo542DT-DNA Succinamopine pSoup(tetR) |
| Product Description | Agrobacterium AGL1(pSoup) strain is C58 with RecA type background, and the nuclear gene contains screening tags - rifampicin resistance gene (rif) and carbenicillin resistance gene (carb). In order to facilitate transformation operation, this strain carries succinine-type Ti plasmid pTiBo542DT-DNA without its own transport function. This plasmid contains the vir gene (the vir gene is a necessary element for T-DNA insertion into the plant genome, and the T-DNA transfer function of the pTiBo542DT-DNA plasmid itself is damaged, but it can help the transferred binary vector T-DNA transfer smoothly). Some expression plasmids, such as pGreen, pGreenII-62SK and pGs2, which were missing replication-related elements of Agrobacterium Tumefaciens (such as REP region of pVS1 replication origin or STA region of pVS1 plasmid), could not reproduce in AGL1 strains. The helper plasmid pSoup can help these incomplete binary expression plasmids replicate in Agrobacterium and make AGL1(pSoup) strains tetracycline (Tet) resistant. The bacterium is suitable for transgenic operation of rice, Arabidopsis and poplar. Agrobacterium AGL1(pSoup) receptive cells were prepared by special technology and detected by pGs2 plasmid. The conversion efficiency was up to 103CFU/μg, and the conversion efficiency did not change after 12 months of storage at -70℃. |
| Unit | Bag |
| Specification | 20*50ul |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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