丁酰胆碱酯酶（BchE）活性检测试剂盒 微量法
Cat.No：BC5975 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Protein ＆ Enzyme Series > Esterase Series > 丁酰胆碱酯酶（BchE）活性检测试剂盒 微量法
Product Information
| Name | Butyrylcholinesterase Activity Assay |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/96S |
Butyrylcholinesterase (BchE, EC3.1.1.8), also known as plasma cholinesterase, pseudocholinesterase, is a serine hydrolase that is synthesized by the liver and enters the blood and is present in almost all animal tissues. BchE is structurally similar to acetylcholinesterase (AchE), but with different substrate specificity and inhibitor sensitivity. Compared with AchE, BchE can effectively hydrolyze larger choline esters, such as butyrylcholine and benzoylcholine, and can remove the toxic effect of nerve agents such as organophosphorus pesticides and carbamate pesticides. Studies have shown that BchE can be an important target for the treatment of Alzheimer's disease.
BchE catalyzed the hydrolysis of butyrylcholine to choline, and the reaction of choline with disulfide p-nitrobenzoic acid (DTNB) to 5-merhydryl-nitrobenzoic acid (TNB). TNB has an absorption peak at 412nm, and BchE activity is calculated by measuring the absorbance increase rate at 412nm.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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