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Elastase

Cat.No：E8211 Solarbio

CAS：39445-21-1

Appearance：Lyophilized powder

Storage：Store at 2-8℃, 1year.

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Product Information

Name	Elastase
CAS	39445-21-1
Molecular Weight	25.9 kDa
Storage	Store at 2-8℃, 1year.
Appearance	Lyophilized powder
Source	Porcine pancrea
Enzyme Activity/Titer	3 units per mg protein
Unit	piece
Specification	25mg 100mg

Elastase is a serine protease that also hydrolyzes amides and esters. It is produced as an inactive zymogen in the pancreas and activated by trypsin in the duodenum.

Product Introduction

Elastase is a serine protease that also hydrolyzes amides and esters. It is produced as an inactive zymogen in the pancreas and activated by trypsin in the duodenum.

Application

Tissue dissociation;

Increase the solubility of membrane protein;

Protein sequence study

Precautions

Poor solubility at neutral pH and concentrations greater than 0.25%. Before dilution into the reaction mixture or medium, it is best to prepare a primary solution in KCl or alkaline buffer to compensate for changes in ionic strength or pH. The pH value is stable at 4.0-10.4.

Unit definition: 1 unit pyrolysis of 1 um of n-succinyl-l-propionyl-l-propionyl-l-alanine-p-nitroaniline at 25℃ and pH 8.0 per minute

International System Classification Number: EC: 4.6.1.19

Optimal pH: 8.5

Isoelectric point: 9.5

Inhibitors: Competitively inhibited by dipeptide derivatives of alanine, valine, leucine and isoleucine; Inhibitors of natural origin (e.g. serpentine); Improved natural inhibitors; Sulfate modified lipids

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.