蛋白酶S,测序级
Cat.No:PQ0131 Solarbio
CAS:66676-43-5
Appearance:Lyophilized powder
Storage:Store at 2-8℃,1 year
Qty:
Size:
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My CartCAS:66676-43-5
Appearance:Lyophilized powder
Storage:Store at 2-8℃,1 year
Qty:
Size:
CAS | 66676-43-5 |
Molecular Weight | 27kDa |
Storage | Store at 2-8℃,1 year |
Appearance | Lyophilized powder |
Source | Staphylococcus aureus |
Enzyme Activity/Titer | ≥500 units per mg dry weight |
Unit | pack |
Specification | 10ug 5×10ug |
Tissue dissociation
Protease S specifically cuts the peptide bond at the end of the -COOH group of aspartate or glutamate.
Application
Study on protein chemistry and peptide atlas
Precautions
Autolysis occurs at temperatures above 40℃. The enzyme was fully active in USP 0.2% SDS. In the presence of an ammonia buffer, the specificity of the enzyme may be limited to glutamyl bonds.
Unit definition: Using casein as substrate, at 37 ℃ and pH 7.8, one unit changes A280nm by 0.001 per minute.
International System Classification Number: EC:3.4.21.19
Optimal pH: 4.0 and 7.8 (with hemoglobin as substrate)
Inhibitors: diisopropyl fluorophosphate (DFP) and univalent anions such as F-, Cl-, Br-, CH3COO- and NO3-
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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