Nuclease
Cat.No：R3980 Solarbio
CAS：37278-25-4
Appearance：Liquid
Storage：Store at -20℃，6 months
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Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
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TGF-β/Smad
Hippo
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Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
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Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | Nuclease |
| CAS | 37278-25-4 |
| Molecular Weight | 34kDa |
| Storage | Store at -20℃，6 months |
| Appearance | Liquid |
| Source | Aspergillus oryzae |
| Unit | piece |
| Specification | 10ku 50ku |
Product Introduction
Nuclease S1 is isolated from certain neurosporus and Aspergillus and exists as a monomer. It hydrolyzes the ends and internal phosphodiester bonds of single-stranded DNA and RNA.
Application
Eliminate unannealed polynucleotide tails and hairpin rings in DNA-RNA or DNA-DNA double strands
Precautions
Zinc ion and calcium ion can activate it.
When diluted with water to ≥6000U/mL, it can be frozen for 6 months;
The diluted solution can be stabilized by adding 0.1% albumin and 10% glycerin.
Unit definition: One unit can hydrolyze 1ug of denatured calf thymus DNA per minute at 37 ℃ and pH 4.6
International System Classification Number: EC: 3.1.30.1
Optimal pH: 4.0-4.6
Inhibitors: EDTA, Citrate, SDS in high concentrations
Product Introduction
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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