Collagenase
Cat.No：C3160 Solarbio
CAS：9001-12-01
Storage：Store at 2-8℃
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Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
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Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | Collagenase |
| CAS | 9001-12-01 |
| Molecular Weight | 68-130 kDa |
| Storage | Store at 2-8℃ |
| Source | Clostridium histolyticum |
| Enzyme Activity/Titer | ≥400 units per milligram dry weight |
| Unit | piece |
| Specification | 10mg 100mg |
Product Introduction
Collagenase is an endopeptidase that digesters natural collagen in the triple helix region. Collagen is the main fibrous component of animal extracellular connective tissue. Bacterial collagenases differ from vertebrate collagenases in that they have a broader substrate specificity. Unlike animal collagenase, which divides collagen in a natural triple helix structure, bacterial collagenase is unique in that it can degrade both natural collagen, which is insoluble in water, and water-soluble denatured collagen. It can attack almost all types of collagen and is capable of making multiple cuts within the triple helix region
Application
Adipocytes, hepatocytes, and cells were isolated from lung, epithelial, and adrenal tissues
Separation of cardiomyocytes from bone, cartilage, muscle, thyroid and endothelial cells
Separation of mammary glands from various other soft tissues
Isolation of human and porcine islet cells
Tissue treated with crude collagenase, whose proteolytic activity is mixed, provides a gentle, selective digestive intercellular matrix with little damage or loss of viability to the cells
AFA collagenase is suitable for applications where the introduction of animal-derived pathogens into bioprocessing procedures needs to be avoided
Precautions
It is grown in a completely animal-free medium and is designed for bioprocessing applications where the introduction of pathogens of animal origin must be prevented.
International System Classification Number: EC: 3.4.24.3
Optimum pH: 6.3-8.5
Isoelectric point: 5.63
Inhibitors: EDTA, EGTA cysteine, histidine, DTT 2-mercaptoethanol o-phenanthroline Hg2+, Pb2+, Cd2+, Cu2+
Unit definition: at 37 ℃, pH 7.5, one unit releases one micromole of L-leucine equivalent from collagen in 5 hours.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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