Deoxyribonuclease I
Cat.No：D8072 Solarbio
CAS：9003-98-9
Appearance：Solution
Storage：Store at -20℃，2 years
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Colloidal Gold Reagent
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Allophycocyanin(APC)-Conjugated
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Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
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Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | Deoxyribonuclease I |
| CAS | 9003-98-9 |
| Storage | Store at -20℃，2 years |
| Appearance | Solution |
| Source | Bovine pancreas |
| Unit | piece |
| Specification | 100u 500U |
Product Introduction
Bovine pancreatic deoxyribonuclease is an endonuclease that preferentially splits phosphodiester bonds adjacent to pyrimidine nucleotides to produce 5' Phosphoric acid terminal polynucleotide, at 3' The site has a free hydroxyl group. Deoxyribonucnase I is secreted by exocrine glands and is highest in the pancreas and parotid glands. It is also found in low amounts in other tissues. This product removes proteases and RNA enzymes and is suitable for RNA recycling or for research where RNA and protein integrity is required. DNase is known to be involved in cell apoptosis and is thought to play a role in the regulation of actin polymerization in cells. In addition to its applications in molecular biology, DNase I is also used to treat cystic fibrosis and systemic lupus erythematosus.
International System Classification Number :EC: 3.1.21.1
Optimum pH:7.8
Isoelectric point :5.08
Inhibitors: 2-mercaptoethanol 2-nitro-5-thiocyanobenzoate actin EGTA sodium dodecyl sulfate calf spleen inhibitor protein carbodiimide and cholesterol sulfate iodoacetate
Unit definition: When pH is 5.0 and 1 unit is used for highly polymerized DNA at 25 ℃, the absorbance at 260nm increases by 0.001/min/ml
Application
DNA removal in primary cell isolation
DNA removal in bioprocessing
Genomic DNA is removed in RT-PCR
In vitro transcription
Protein and nucleic acid purple diplomatic link
radiolabelling
Unit definition
At a pH of 5.0, when 1 unit is used for highly polymerized DNA at 25℃, the absorbance at 260nm increases by 0.001/min/ml
Precautions:Avoid repeated freezing and thawing
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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