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谷胱甘肽还原酶活性系数（GRAC）检测试剂盒微量法

Cat.No：BC6005 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Name	Glutathione Reductase Activation Coefficient Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Unit	Kit
Specification	100T/48S

Glutathione Reductase (GR) is a flavin protein REDOX reductase widely found in eukaryotes and prokaryotes. The enzyme uses riboflavin adenine dinucleotide (FAD), a riboflavin derivative, as a cogroup. Catalyzed NADPH reduction oxidized glutathione (GSSG) to produce reduced glutathione (GSH), maintaining sulfhydryl group and membrane protein in reduced state. When riboflavin was lacking in vivo, FAD content decreased correspondingly, GR activity also decreased, glutathione reductase activity Coefficient (GRAC) increased rapidly, and clinical symptoms such as cheilitis, keratitis, conjunctivitis appeared. Therefore, the use of GRAC in the evaluation of riboflavin nutritional status is of great significance for early detection and prevention of patients with deficiency.

GR catalyzes NADPH reduction of GSSG to produce GSH, which reacts with 5,5 '-dithiobis-2-nitrobenoic acid (5,5' -dithiobis-2-nitrobenoic acid, DTNB) to produce 2-nitro-5-mercaptobenic acid. 2-nitro-5-mercaptobenzoic acid has a characteristic absorption peak at 412nm, and the activity of GR can be calculated by the change of absorbance at 412nm. According to the change of GR activity before and after adding FAD, GRAC can be calculated.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.