QuickCut restriction endonuclease Agel
Cat.No：Q2460 Solarbio
Storage：Store at -20℃，2 years.
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> Molecular Biology > Molecular Cloning-Related Enzymes > Endonuclease > QuickCut restriction endonuclease Agel
Product Information
| Name | QuickCut restriction endonuclease Agel |
| Storage | Store at -20℃，2 years. |
| Grade | 1rxn/ul |
| Unit | Piece |
| Specification | 25rxns |
Isomerases, AsiGI, CspAI, BshTI, PinAI Note: Isomerases may have different sensitivities to different methylation modifications. Recognition site: ACCGGT; 5 '... A ↓ C C G G T... 3'; 3 '... T G G C C ↑ A... 5' Product Description: LightNing fast endonuclease is a series of genetically engineered fast restriction endonuclease, which is suitable for fast enzyme cutting of plasmid DNA, PCR products or genomic DNA. All LightNing fast endonuclease enzymes have excellent activity in the universal CutOne or CutOne Color Buffer, and can complete the enzyme digestion in 5 to 15 minutes. In addition, the dephosphorylation and linking reagents are 100% active in the CutOne Buffer, supporting a tubeization reaction and improving the "enzyme-reduction-modified-linking" experience. The CutOne Color Buffer includes red and yellow tracer dyes that allow the product to be used directly for gel electrophoresis. The migration rate of the red dye in CutOne Color Buffer was close to that of 2500 bp double-stranded DNA fragment in 1% agarose gel. The migration rate of yellow dye and 10 bp double-stranded DNA fragment in 1% agarose gel is close. Characteristics and Usage: Recommended reaction conditions: 1× CutOne buffer; Incubation at 37℃; The reaction system was formulated according to "DNA Rapid enzyme digestion Process". Inactivation condition: incubated at 80℃ for 20 min. Methylation sensitivity: For DNA methylated by EcoKI, clipping may be blocked.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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