Methylcitrate Synthase（MCS）Activity Assay Kit
Cat.No：BC5865 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Tricarboxylic Acid Cycle Series > Methylcitrate Synthase（MCS）Activity Assay Kit
Product Information
| Name | Methylcitrate Synthase（MCS）Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
Methyl citrate synthase (MCS) is widely found in the mitochondrial matrix of animals, plants, microorganisms and cultured cells, and is involved in the regulation of the tricarboxylic acid cycle together with citrate synthase (CS).
Measurement Principle：
MCS catalyzes the production of methyl citric CoA by propionyl CoA and oxaloacetate, which is further hydrolyzed to produce methyl citric acid; This reaction results in the conversion of colorless DTNB to yellow TNB with a characteristic absorption peak at 412 nm, from which MCS activity can be calculated.
Self Provided：
Visible spectrophotometer/microplate reader, microglass cuvette/96-well plate, balance, cryogenic centrifuge, water bath, mortar/homogenizer/cell sonicator, ice, and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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