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Pyruvate Phosphate Dikinase（PPDK）Activity Assay Kit

BC5855Manual

Cat.No：BC5855 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Name	Pyruvate Phosphate Dikinase（PPDK）Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Recommend	If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602
Unit	Kit
Specification	100T/96S

Determination of Significance：

"pyruvate phosphate dikinase (PPDK, EC 2.7.9.1) is a rate-limiting enzyme of the C4 pathway and the sedum acid metabolism pathway, which catalyzes the three-step reaction of ATP, pyruvate and Pi to produce phosphoenolpyruvate. This enzyme is mainly found in the chloroplast matrix of C4 plants and has an important regulatory role in photosynthetic function.

Measurement Principle：

The reverse reaction of PPDK catalyzes the generation of pyruvate by phosphoenolpyruvate (PEP), AMP and PPi to pyruvate, ATP and Pi, and lactate dehydrogenase (LDH) further catalyzes the generation of lactate and NAD+ by pyruvate and NADH, and the rate of NADH reduction is measured at 340 nm, from which PPDK activity can be calculated.

Self Provided：

UV spectrophotometer/microplate reader, microquartz cuvette/96-well UV plate, balance, cryogenic centrifuge, water bath, mortar/homogenizer, ice, and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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