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Total Protein Thiol Content Assay Kit

BC5800Manual

Cat.No：BC5800 Solarbio

Storage：Store at 2-8℃，6 months

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Product Information

Reagents Required But Not Provided	Please check the manual first, there are some reagents you should prepare by yourself.
Name	Total Protein Thiol Content Assay Kit
Detection Method	Spectrophotometer
Storage	Store at 2-8℃，6 months
Unit	Kit
Specification	50T/48S

Determination of Significance：

The presence of sulfhydryl allows proteins to undergo disulfide bond formation, thereby maintaining molecular stability and functionality. Sulfhydryl is also involved in redox reactions and have important biological roles. In cells, changes in sulfhydryl content are closely related to the onset and progression of a variety of diseases, and thus sulfhydryl have become an important object of study in the biomedical field. This kit determines the sum of sulfhydryl produced by protein disulfide bond breakage and its own free sulfhydryl.

Measurement Principle：

Reducing agent will cause disulfide bond cleavage to generate sulfhydryl, sulfhydryl will undergo nucleophilic reaction, that is, sulfhydryl reacts with 5,5'-dithio-bis-nitrobenzoic acid (DTNB) to produce a yellow color compound, with a maximum absorption peak at 412 nm, from which the total sulfhydryl content of proteins can be calculated.

Self Provided：

Spectrophotometer, tabletop centrifuge, water bath, adjustable pipette, 1 mL glass cuvette, Acetone, mortar/homogenizer/ sonicator and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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