Protein Disulfide Bond Content Assay Kit
Cat.No：BC5795 Solarbio
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > REDOX Series > Antioxidant Series > Protein Disulfide Bond Content Assay Kit
Product Information
| Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
| Name | Protein Disulfide Bond Content Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
Proteins are an important class of biomolecules found in all living organisms and are the basic substances of life. Disulfide bond is a chemical bond that connects the sulfhydryl groups of two different cysteine residues in different peptide chains or the same peptide chain. Disulfide bonds have a great influence on the structure of proteins and play a role in stabilizing the spatial structure of peptide chains in protein molecules, so it is particularly important to determine the content of protein disulfide bonds.
Measurement Principle：
Reducing agent will make the disulfide bond cleaved, the cleaved sulfhydryl group will undergo nucleophilic reaction, that is, the sulfhydryl group reacts with 5,5'-dithio-bis-nitrobenzoic acid (DTNB) to produce a yellow color compound, with a maximum absorption peak at 412 nm, according to which, we can calculate the content of the disulfide bond of the protein.
Self Provided：
Spectrophotometer/microplate reader, tabletop centrifuge, water bath, adjustable pipette, Micro glass cuvettes/96 well plates, acetone, mortar/homogenizer/ sonicator and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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