O.C.T. Compound
Cat.No：4583 进口
Storage：Store at RT
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Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
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MAPK
NF-κB
Membrane Transporter & Ion Channel
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Hippo
Antibody-drug Conjugate/ADC Related
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
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Product Information
| Name | O.C.T. Compound |
| Storage | Store at RT |
| Unit | Bottle |
| Specification | 118ml/瓶 118ml/瓶，12瓶/箱 |
OCT Compound is a water-soluble mixture of polyethylene glycol and polyvinyl alcohol and has been widely used in immunohistochemistry laboratories, it can support the tissue to increase its continuity and reduce wrinkles and fragmentation. Because OCT Compound is water-soluble, it will be soluble in water when bleach, so the background staining will not be increased in the later staining.Pre-infiltrating tissue by OCT Compound (opti-mum cutting temperature compound), then slice in cryostat .
Procedure:
1.take the tissue from sucrose solution, put it in mixture solution which 20g/L sucrose and OCT compound volume ratio is 1:1.
2. Transfer to OCT compound for 4h at room temperature, replace with new OCT compound and soak at room temperature for 6h.
3.Put the specimen on a tray, secice in a cryostat (generally is 22℃), thick is 22um, stick on pre-treated slides and store in-20℃ refrigerator.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Click to check >>Author：Pengshan Cai, Teng Cai, Xiaobin Li, Lei Fan, Guang
IF：3.0610
Publish_to：American journal of Translation research
PMID：30899386
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