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Dihydroflavonol Reductase (DFR) Activity Assay Kit

BC5785Manual

Cat.No：BC5785 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Reagents Required But Not Provided	Please check the manual first, there are some reagents you should prepare by yourself.
Name	Dihydroflavonol Reductase (DFR) Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Recommend	If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602
Unit	Kit
Specification	100T/96S

Determination of Significance：

Dihydroflavonol-4-reductase (DFR) is a key enzyme in the biosynthetic pathway of phycocyanin, which catalyzes the production of different anthocyanin precursors from dihydroflavonols. Therefore, DFR determines the type and content of anthocyanins in plants, which in turn affects the color of the tissues or organs of plants.

Measurement Principle：

DFR, NADPH and dihydroflavonols react to produce anthocyanin precursors. NADPH has a maximum absorption peak at 340 nm. The magnitude of DFR activity can be obtained by detecting the rate of change of NADPH at 340 nm.

Self Provided：

Ultraviolet Spectrophotometer/ microplate reader, water bath, benchtop centrifuge, balance, adjustable pipettes, micro quartz cuvette/96 well UV plate, mortar and homogenizer, anhydrous ethanol (AR), ammonium sulfate (AR), ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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