Dihydroflavonol Reductase (DFR) Activity Assay Kit
Cat.No：BC5780 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > REDOX Series > Antioxidant Series > Dihydroflavonol Reductase (DFR) Activity Assay Kit
Product Information
| Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
| Name | Dihydroflavonol Reductase (DFR) Activity Assay Kit |
| Detection Method | Spectrophotometer |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 50T/48S |
Determination of Significance：
Dihydroflavonol-4-reductase (DFR) is a key enzyme in the biosynthetic pathway of phycocyanin, which catalyzes the production of different anthocyanin precursors from dihydroflavonols. Therefore, DFR determines the type and content of anthocyanins in plants, which in turn affects the color of the tissues or organs of plants.
Measurement Principle：
DFR, NADPH and dihydroflavonols react to produce anthocyanin precursors. NADPH has a maximum absorption peak at 340 nm. The magnitude of DFR activity can be obtained by detecting the rate of change of NADPH at 340 nm.
Self Provided：
Ultraviolet Spectrophotometer, water bath, benchtop centrifuge, balance, adjustable pipettes, 1mL quartz cuvette, mortar and homogenizer, anhydrous ethanol (AR), ammonium sulfate (AR), ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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