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My CartReactivity：Nicotiana tabacum,Triticum aestivum,Glycine max,Arabidopsis thaliana
Application： ELISA,WB
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Host：Rabbit
Qty：
Size：
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:2000-5000. |
| Target | ATG7 |
| Background | E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes. Involved in the senescence process (PubMed:12070171). |
| Swiss Prot | Q94CD5 |
| Gene ID | 834630 |
| Subcellular Location | Cytoplasm |
| Immunogen | A synthetic peptide of Arabidopsis thaliana ATG7 |
| Calculated MW | 77kDa |
| Observed MW | 85kDa |
| Purification Method | Serum |
| Storage Buffer | Buffer: PBS with 50% Glycerol, 0.03% Proclin300, 0.5% BSA, pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:2000-5000. ELISA 1:3000-30000. |
| Specification | 100ul |
| Reactivity | Nicotiana tabacum Triticum aestivum Glycine max Arabidopsis thaliana |
| Application | WB ELISA |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with ATG7 antibody diluted at 1:3000;Lane: Glycine max,Triticum aestivum,Nicotiana tabacum.
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