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CAS：130100-20-8
Storage：Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：≥95%
Appearance：Solid
Qty：
Size：
| CAS | 130100-20-8 |
| Name | Mag-Fura-2 AM |
| Molecular Formula | C30H30N2O19 |
| Molecular Weight | 722.56 |
| Solubility | Soluble in DMSO |
| Purity | ≥95% |
| Appearance | Solid |
| Storage | Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| MDL | MFCD07437966 |
| SMILES | CC(=O)OCOC(=O)CN(CC(=O)OCOC(=O)C)C1=C(C=C2C=C(OC2=C1)C3=NC=C(O3)C(=O)OCOC(=O)C)OCC(=O)OCOC(=O)C |
| InChIKey | PCDUVTXECKTHBH-UHFFFAOYSA-N |
| InChI | InChI=1S/C30H30N2O19/c1-16(33)42-12-46-26(37)9-32(10-27(38)47-13-43-17(2)34)21-7-22-20(5-23(21)41-11-28(39)48-14-44-18(3)35)6-24(50-22)29-31-8-25(51-29)30(40)49-15-45-19(4)36/h5-8H，9-15H2，1-4H3 |
| PubChem CID | 131176 |
| Background | Mag Fura-2 AM is a ratio type Mg2+/Ca2+fluorescence probe that can be excited by ultraviolet light (Ex=334-360 nm， Em=510 nm) and has membrane permeability. |
| Unit | Bottle |
| Specification | 50ug |
Mag-Fura-2 AM is an intracellular magnesium ion indicator, which can also be used to detect calcium ion concentration, and is a ratio probe excited by ultraviolet. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue transition from 369nm to 330nm. Mag-Fura-2 AM is permeable to cell membranes and can be loaded into cells after simple incubation.
Fluorescence microscope (for reference only)
Excitation Fura 2 filter set
Emission Fura 2 filter set
Recommended plate Black wall/clear bottom
Fluorescence microplate reader (for reference only)
Excitation 340, 380
Emission 510
Cutoff 475
Recommended plate Black wall/clear bottom
Instrument specification(s) Bottom read mode/Programmable liquid handling
Example Protocol (for reference only)
Mag-Fura-2 AM Working Solution
For most cell lines, Mag-Fura-2 AM at a final concentration of 4 to 5 μM is recommended. The exact concentration of indicators required for cell loading must be determined empirically.
If it is necessary to prepare a stock solution, it is recommended to store in aliquots to avoid product failure caused by repeated freezing and thawing.
Note If your cells contain organic anion-transporters, probenecid (1-2 mM) may be added to the dye working solution (final in well concentration will be 0.5-1 mM) to reduce leakage of the de-esterified indicators.
SAMPLE EXPERIMENTAL Protocol (for reference only)
Following is our recommended protocol for loading AM esters into live cells. This protocol only provides a guideline and should be modified according to your specific needs.
Prepare cells in growth medium overnight.
On the next day, add 1X Mag-Fura-2 AM working solution into your cell plate.
Note If your compound(s) interfere with the serum, replace the growth medium with fresh HHBS buffer before dye-loading.
Incubate the dye-loaded plate in a cell incubator at 37 ℃ for 30 to 60 minutes.
Note Incubating the dye for longer than 1 hour can improve signal intensities in certain cell lines.
Replace the dye working solution with HHBS or buffer of your choice (containing an anion transporter inhibitor, such as 1 mM probenecid, if applicable) to remove any excess probes.
Add the stimulant as desired and simultaneously measure fluorescence using either a fluorescence microscope equipped with a Fura 2 filter set or a fluorescence plate reader containing a programmable liquid handling system such as a FlexStation, at Ex/Em1 = 340/510 nm cutoff 475 nm and Ex/Em2 = 380/510 nm cutoff 475 nm.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
