Soil Acidic xylanase (S-ACX）Activity Assay Kit
Cat.No：BC5725 Solarbio
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > Other Biochemical Series > Soil Series > Soil Acidic xylanase (S-ACX）Activity Assay Kit
Product Information
| Name | Soil Acidic xylanase (S-ACX）Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
Soil acid xylanase (S-ACX), also known as soil acidic hemicellulase, is mainly isolated from microorganisms with an optimal growth pH of 4-5.
Measurement Principle：
In acidic environments, S-ACX catalyzes the degradation of xylan into reducing oligosaccharides and monosaccharides. Under boiling water bath conditions, it further undergoes a color reaction with 3,5-dinitrosalicylic acid, with a characteristic absorption peak at 540nm. The color depth of the reaction solution is directly proportional to the amount of reducing sugars produced by enzymatic hydrolysis. By measuring the rate of increase in absorbance of the reaction solution at 540nm, S-ACX activity can be calculated.
Self Provided：
Spectrophotometer/Microplate reader, micro glass cuvette/96 well plate, balance, desk centrifuge, water bath, 30-50 mesh sieve, distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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