Preactivated PerCP-Cy5.5 Maleimide
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Cat.No：IC8830 Solarbio
Storage：Store at -20℃，2 years
Purity：≥95%
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PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
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MAPK
NF-κB
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Hippo
Antibody-drug Conjugate/ADC Related
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | Preactivated PerCP-Cy5.5 Maleimide |
| Molecular Formula | No data available |
| Molecular Weight | ~35 kDa |
| Purity | ≥95% |
| Storage | Store at -20℃，2 years |
| Background | Preactivated PerCP-Cy5.5 Maleimide is a sulfhydryl reactive dye that reacts with free sulfhydryl groups on proteins. The activated dye binds easily to the protein or antibody without changing the spectral characteristics of PerCP-Cy5.5. Ex/Em = 489/679 nm. |
| Unit | Bottle |
| Specification | 1mg |
Usage (IgG as an example, for reference only)
IgG reduction
For better labeling, the antibody solution is recommended at 1 mg/mL, and if it is lower than 1 mg/mL, the antibody should be concentrated. Antibody reduction can be performed in phosphate buffers. The type, concentration and incubation time of reducing agent can refer to the corresponding literature. After purification, the concentration of the reducing antibody should be determined.
Note:
Reducing antibodies are unstable and it is best to conduct the coupling reaction immediately after purification.
Marking steps (for reference only)
1. The reduced antibody is added to the pre-activated dye tube at the ratio of 0.5:1 (mass ratio).
Note:
the pre-activated PerCP-Cy5.5 dye 1 mg specification is recommended for one-time use, that is, if the concentration of antibody solution is 1 mg/mL, according to the mass ratio of 0.5:1, 2000 μL of antibody solution is required to be added to the pre-activated dye.
2. Shake and incubate at room temperature (20-25℃) for 4 h away from light.
3. Block the free sulfhydryl group on the antibody, need to refer to the literature.
4. The antibody /PerCP-Cy5.5 conjugate solution was purified by volumetric exclusion chromatography.
Note:
If long-term storage, need to add antibody preservation solution.
Matters needing attention
1. To label different antibodies, the required dye and antibody labeling ratio may be different. When the antibody quality, labeling steps and other factors that may affect the labeling result are excluded, the antibody quality can be reduced to achieve a better labeling effect.
2. Fluorescent dyes are all quenched, please pay attention to avoid light to slow down fluorescence quenching.
3. For your safety and health, please wear a lab coat and disposable gloves.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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