Lysosome dark red fluorescent probe
Cat.No：IF1830 Solarbio
Storage：Store at -20℃，2 years
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> Small Molecule Compounds > Star Products > Star Dyes & Probes > Lysosome dark red fluorescent probe
Product Information
| Name | Lyso-Tracker Deep Red |
| Storage | Store at -20℃，2 years |
| Background | It is a fluorescent probe for specific labeling of lysosomes in living cells， which can be selectively retained in more acidic lysosomes， so as to achieve specific fluorescent labeling of lysosomes. Suitable for staining of living cells， but not suitable for staining of fixed cells. EX/EM(nm)=649/665. |
| Unit | Bottle |
| Specification | 50ul |
Item number Product concentration Ex/Em save
IF1830 Lysosomal dark red fluorescent probe 1 mM 649/665-20 ℃,2 years
IF1840 Lysosomal red fluorescent probe 1 mM 577/590-20 ℃,2 years
IF1850 Lysosome green fluorescent probe 1 mM 504/511-20 ℃,2 years
Usage (for reference only)
1. Preparation of lysosomal probe working solution
(1) A small amount of lysosomal probe reserve solution (1mM) was added into the cell culture solution at the ratio of 1:10,000-1:20,000, so that the final concentration was 50-100 nM, and the lysosomal probe working solution was formed after mixing.
(2) The lysosomal probe working solution can be pre-warmed at 37℃ before use.
2. Fluorescent labeling of lysosomes
(1) The cell medium was removed, and after 1×PBS was cleaned once, the lysosomal probe working solution prepared in Step 1 was added, and the cells were incubated at 37℃ for 30 min and 2 h. The incubation time of different cells was different, and it was recommended to adjust according to the staining effect.
(2) The lysosomal probe working solution was removed, and after three times of cleaning with 1×PBS, the fluorescence microscope was photographed for observation. If it is necessary to add Hoechst 33342 to re-stain, it is recommended to use Hoechst 33342 with a concentration of 10 μg/mL and incubate at 37℃ for 5 min to remove the dye. Take pictures after 1×PBS cleaning.
Matters needing attention
1. If the staining effect is not good, the concentration of the lysosomal probe can be increased, or the dyeing time can be extended appropriately within the recommended time range.
2. In order to reduce the dyeing background, use a lower concentration of dye as much as possible.
3. Take pictures quickly, because the dye is easy to quench with the increase of the photo time.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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