6-CDCFDA SE
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Cat.No：ICA10030 Solarbio
Storage：Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：≥98%
Appearance：Soild
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PI3K/Akt/mTOR
Endocrinology & Hormones
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HIF
Immunology & Inflammation
Stem Cells
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Oscillating Mixing & Stirring & Dispersing Instrument
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Spectrophotometer
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Product Information
| Name | 6-CDCFDA SE |
| Molecular Formula | C29H17Cl2NO11 |
| Molecular Weight | 626.4 |
| Solubility | Soluble in DMSO |
| Purity | ≥98% |
| Appearance | Soild |
| Storage | Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| Background | 6-CDCFDA is a live cell fluorescent tracer probe that can enter cells through passive diffusion and covalently bind to intracellular proteins. Once inside the cell， the non-fluorescent 6-CDCFDA is hydrolyzed by intracellular esterase to produce green fluorescence. |
| Unit | Bottle |
| Specification | 5mg |
6-CDCFDA is a live cell fluorescent tracer probe, which can enter cells through passive diffusion and covalently bind to intracellular proteins, and is a long-acting cell tracer dye. Once inside the cell, the non-fluorescent 6-CDCFDA is hydrolyzed by lactase to produce green fluorescence. These fluorescent products can only accumulate in cells with an intact cell membrane, so dead cells without an intact cell membrane cannot be stained. 6-CDCFDA is not sensitive to pH changes and can be fixed by formaldehyde or glutaraldehyde.
Fluorescein diacetate (FDA) and its derivatives are non-fluorescent molecules that diffuse into cells and are hydrolyzed by intracellular non-specific esterases to produce fluorescent products. Fluorescent products can only accumulate in cells with intact cell membranes. As a result, dead cells are not stained. The dynamics of membrane transport and intracellular hydrolysis of FDA and its analogues, such as CDCFDA, are related to cell function, so FDA labeling can be used to monitor cells by flow cytometry or fluorescence microscopy. The fluorescence intensity of FDA-dye-labeled cells varied widely between cell lines and strains, possibly due to differences in cellular lactase activity.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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