Diphenyl Blue
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Cat.No：ID5110 Solarbio
CAS：72-57-1
Storage： Store at RT，2 years.
Purity：>80%（Dye content）
Appearance：Light brown to black Solid
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PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
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ER Stress
Cell Cycle
Cytoskeleton
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Ubiquitin
JAK/STAT
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
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Product Information
| CAS | 72-57-1 |
| Name | Diphenyl Blue |
| Molecular Formula | C34H24N6Na4O14S4 |
| Molecular Weight | 960.81 |
| Solubility | Soluble in Water |
| Purity | >80%（Dye content） |
| Appearance | Light brown to black Solid |
| Storage | Store at RT，2 years. |
| MDL | MFCD00003969 |
| Background | It is an anionic dye，which is one of the most commonly used methods to identify dead cells in tissue and cell culture. Living cells do not get dyed blue; And dead cells can be dyed blue. |
| Unit | Bottle |
| Specification | 100mg 1g 5g |
Trypan Blue is an anionic dye. Trypan blue can not penetrate the normal cell membrane of living cells, so living cells are not colored; The increased permeability of the cell membrane of dead cells can make dyes enter the cell through the cell membrane and make the dead cells colored blue, which is the most commonly used method to detect cell viability. The method could not distinguish between necrotic cells and apoptotic cells. When the cell is inactive or the cell membrane is incomplete, Trypan blue can stain it blue. Normal living cells have a complete cell membrane structure and can reject Trypan blue, and the cell will not be dyed blue. However, macrophages can phagocytose trypan blue, so it can be used as a living stain for macrophages.
Instructions (for reference only) :
1. Cell collection: Digest the adherent cells with pancreatic enzymes and/or EDTA, and the suspended cells can be collected directly. When the cells were collected, they were centrifuged at 1000-2000 rpm for 1 min, the supernatant was discarded, and the single-cell suspension was prepared and diluted appropriately.
2. Trypan Blue staining: Cell suspension is mixed with 0.4% Trypan blue solution at 9:1 ratio (so that the final concentration of Trypan blue is 0.04%), and the staining time is 3 minutes (generally, the dyeing time of 3 minutes is enough, and the dyeing time can be longer, but it should not exceed 10 minutes).
3. Cell count: Take an appropriate amount of stained cells and count them with a blood cell count plate. (Dead cells are blue and swollen, dull; Living cells do not stain and remain in their normal form, shiny.) Cell survival rate (%) = Total number of living cells/(total number of living cells + total number of dead cells) ×100%
Note:
1. The dyeing time should not be too long, otherwise living cells will gradually accumulate dye and dye color, so that the detection result is low.
2. If there is precipitation in the dye solution before dyeing, filter and remove the precipitation before use.
3. Trypan Blue is a potential carcinogenic hazard. For your safety and health, please wear a lab coat and disposable gloves.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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