DAN
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Cat.No：ID3930 Solarbio
CAS：771-97-1
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：≥98%
Appearance：Solid
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PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| CAS | 771-97-1 |
| Name | DAN |
| Molecular Formula | C10H10N2 |
| Molecular Weight | 158.2 |
| Solubility | Soluble in DMSO ≥5mg/mL(Need ultrasonic) |
| Purity | ≥98% |
| Appearance | Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| MDL | MFCD00004116 |
| SMILES | C1=CC=C2C=C(C(=CC2=C1)N)N |
| InChIKey | XTBLDMQMUSHDEN-UHFFFAOYSA-N |
| InChI | InChI=1S/C10H10N2/c11-9-5-7-3-1-2-4-8(7)6-10(9)12/h1-6H，11-12H2 |
| PubChem CID | 69872 |
| Background | DAN is a fluorescent probe used to detect nitric oxide (NO) production in living cells and animals. Ex (nm) : 364/Em (nm) : 406 |
| Biological Activity | 2，3 Diaminonaphthalene is a highly selective colorimetric and fluorometric reagent for selenium detection and also used for the fluorometric determination of nitrite. |
| In Vitro | Poly(2，3-diaminonaphthalene) microspheres as a novel quencher for fluorescence-enhanced nucleic acid detection[1]. 2，3-Diaminonaphthalene (DAN) is possibly a carcinogenic reagent[2]. |
| Kinase Experiment | Various concentrations of each drug are incubated for 60 min at 37°C in OS-mL reaction volumes containing PBS (pH 7.4)， 200 μM 2，3-Diaminonaphthalene (DAN) and 40 μM Sp/NO. Spermine/NO will spontaneously decompose at pH7.4 to produce 2 mol of NO and 1 mol of spermine with a half-life of 39 min at 37°C. Following the incubation period， 2.5 mL of 10 mM NaOH is added to each tube to stop the reaction. The NO-dependent N-nitrosation of 2，3-Diaminonaphthalene to yield its highly fluorescent N-nitrosated derivative， 2，3-naphthotriazole， is quantified by measuring the fluorescence of each sample using an excitation wavelength of 375 nm and an emission wavelength of 450nm. The concentration of Triazole is determined using standards of the pure 2，3-naphthotriazole[3]. |
| Data Literature Source | [1]. Tian J，et al. Poly(2，3-diaminonaphthalene) microspheres as a novel quencher for fluorescence-enhanced nucleic acid detection. Analyst. 2011 Jun 7;136(11):2221-4. [2]. Martínez-Tomé MJ，et al. Immobilization and characterization of 2，3-diaminonaphthalene/cyclodextrin complexes in a sol-gel matrix: a new fluorimetric sensor for nitrite. J Fluoresc. 2009 Jan;19(1):119-25. [3]. Grisham MB，et al. Effects of aminosalicylates and immunosuppressive agents on nitric oxide-dependent N-nitrosation reactions. Biochem Pharmacol. 1994 May 18;47(10):1897-902. |
| Unit | Bottle |
| Specification | 50mg 100mg |
DAN是一种荧光探针，用于检测活细胞和动物体内的一氧化氮（NO）产生。 DAN与NO的反应产物是具有蓝色荧光的三唑。基于DAN的NO评估方法是方便的并且适用于体内和体外的许多生命系统。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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