Glycosylated Hemoglobin (GHb) Content Assay Kit
Cat.No：BC5610 Solarbio
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycometabolic Series > Glycosylated Hemoglobin (GHb) Content Assay Kit
Product Information
| Name | Glycosylated Hemoglobin (GHb) Content Assay Kit |
| Detection Method | Spectrophotometer |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 50T/48S |
Determination of Significance：
Glycated hemoglobin (GHb) is formed when a non-enzyme reaction occurs between glucose in the serum and hemoglobin in the erythrocyte. The amount of GHb generated is proportional to the mean blood glucose during the 8–10 weeks before the test. GHb is composed of HbA1a, HbA1b and HbA1c. HbA1c accounts for about 70 % and its structure is relatively stable. GHb content is used as a biomarker for diabetes mellitus control.
Measurement Principle：
The hexose moiety of GHb is converted to 5-hydroxymethylfurfural (5-HMF) by heating at 100℃ in the presence of a weak acid. 5-HMF reacts with Thiobarbituric Acid (TBA) to produce colored compound, which has an absorption peak at 443 nm.
Self Provided：
Spectrophotometer, desk centrifuge, balance, water-bath, transferpettor, 1mL glass cuvette, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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