Free Hemoglobin ( FHb ) Content Assay Kit
Cat.No：BC5590 Solarbio
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > Other Biochemical Series > Other Series > Free Hemoglobin ( FHb ) Content Assay Kit
Product Information
| Name | Free Hemoglobin ( FHb ) Content Assay Kit |
| Detection Method | Spectrophotometer |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 50T/48S |
Determination of Significance：
Red blood cells rupture and free hemoglobin (FHb) content increases in the plasma when hemolysis occurs in blood vessels. Plasma FHb concentration increases mildly to moderately in patients with autoimmune hemolytic anemia, sickle cell disease or thalassemia. The patients have hematuria symptoms who suffer from paroxysmal nocturnal hemoglobinuria, paroxysmal cold hemoglobinuria or unstable hemoglobin disease. It is important to detect FHb content for diagnosis of hemolytic diseases.
Measurement Principle：
Heme in hemoglobin has the catalytic function of peroxidase analogues. It could catalyze the oxidation of 4-aminoantipyrine and phenyl amines by H2O2 to form purple quinones. It has a characteristic absorption peak at 546 nm, and its color depth is directly proportional to FHb content.
Self Provided：
Spectrophotometer, desk centrifuge, constant temperature foster box/water-bath, transferpettor, 1mL glass cuvette, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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