Angiotensin Converting Enzyme Inhibitor Activity Aassay Kit
Cat.No：BC5575 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Other Biochemical Series > Other Series > Angiotensin Converting Enzyme Inhibitor Activity Aassay Kit
Product Information
| Name | Angiotensin Converting Enzyme Inhibitor Activity Aassay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Angiotensin Converting Enzyme (ACE, EC 3.4.15.1, also called ACE1) is a zinc-containing peptidyl dipeptide hydrolase with a calculated molecular mass of 120-150 kDa. ACE is widely detected in the endothelial cell of organ tissues included lung, brain, kidney. ACE catalyzes the formation of angiotensin I to angiotensin II, which could cause vasoconstriction, promote synthesis and release of adrenocortical hormone aldosterone. ACE inhibitors could reduce the production of angiotensin II and increase bradykinin activity, which is helpful for the treatment of hypertension, cardiac hypertrophy, heart failure and other diseases.
Measurement Principle：
ACE could catalyze FAPGG to form FAP and glycylglycine. ACE inhibitors could reduce the hydrolysis of FAPGG by inhibiting ACE activity. FAPGG has a characteristic absorption peak at 340 nm, and ACE inhibitor activity is calculated by measuring the rate of decrease in absorbance at 340nm.
Self Provided：
Spectrophotometer/Microplate reader, desk centrifuge, balance, transferpettor, constant temperature foster box/water-bath, micro quartz cuvette/96 well UV plate, mortar/homogenizer/cell ultrasonic crusher, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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