BCECF AM(pH Fluorescent probe,5mM)
Cat.No:B3021 Solarbio
Storage:Store at -20℃,avoid light
CAS:117464-70-7
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My CartStorage:Store at -20℃,avoid light
CAS:117464-70-7
Qty:
Size:
Name | BCECF AM |
CAS | 117464-70-7 |
Molecular Formula | Mixture(C30H20O11, C35H28O15, C40H36O19) |
Molecular Weight | Average 688.6 |
Storage | Store at -20℃,avoid light |
Unit | Bag |
Specification | 50ul |
fluorescence Spectrum:
Ex/Em: 505/520 nm
Product Introduction:
BCECF, AM is a fluorescent dye that can penetrate the cell membrane to detect intracellular pH. BCECF, AM has no fluorescence and upon entry into cells can be cleaved by intracellular esterase to form BCECF, thus being retained in cells. BCECF can be excited to form green fluorescence under appropriate pH conditions. The maximum excitation wavelength and emission wavelength are different depending on the pH, the maximum excitation wavelength is about 503 nm, the maximum emission wavelength is about 520 nm, and the excitation wavelength is 488 nm and the emission wavelength is 535 nm recommended for practical detection.
Uses:
BCECF, AM are widely used not only in the study of mammalian cells, but also in the in vivo pH level measurement of animal tissues, plant cells, bacteria and yeast. In addition, BCECF, AM are also widely used in the process of cytotoxicity, apoptosis, cell adhesion, drug resistance, cell chemotaxis and other processes with intracellular pH changes.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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