Lysosomal protein extraction kit

The lysosome is surrounded by a unit membrane containing a variety of hydrolase, including protease, nucleic acid degrading enzyme and glycosidase. Its main function is the digestion of intracellular substances. In addition, lysosomes are closely related to organ formation, hormone secretion regulation and the occurrence of certain diseases. Lysosomal protein extraction kit provides a complete set of reagents to extract total lysosomal protein from a variety of primary or pass-through cells and a variety of solid tissues, such as brain, spinal cord, nerve junction or fiber, fat, liver, digestive tract, kidney, heart, muscle, blood vessel, connective tissue and other animal tissues. This kit contains a unique formulation that effectively dissolves membrane components. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extracted from this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gel shift gel blocking experiment, enzyme activity determination, etc. The protein extracted by this kit is an active protein with natural protein conformation. The lysosome is surrounded by a unit membrane containing a variety of hydrolase, including protease, nucleic acid degrading enzyme and glycosidase. Its main function is the digestion of intracellular substances. In addition, lysosomes are closely related to organ formation, hormone secretion regulation and the occurrence of certain diseases. Lysosomal protein extraction kit provides a complete set of reagents to extract total lysosomal protein from a variety of primary or pass-through cells and a variety of solid tissues, such as brain, spinal cord, nerve junction or fiber, fat, liver, digestive tract, kidney, heart, muscle, blood vessel, connective tissue and other animal tissues. This kit contains a unique formulation that effectively dissolves membrane components. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extracted from this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gel shift gel blocking experiment, enzyme activity determination, etc. The protein extracted by this kit is an active protein with natural protein conformation.

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Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.