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Angiotensin Converting Enzyme Activity Assay Kit

BC5545Manual

Cat.No：BC5545 Solarbio

Storage：Store at 2-8℃，6 months

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Product Information

Name	Angiotensin Converting Enzyme Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at 2-8℃，6 months
Recommend	If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602
Unit	Kit
Specification	100T/96S

Determination of Significance：

Angiotensin Converting Enzyme (ACE, EC 3.4.15.1, also called ACE1) is a zinc-containing peptidyl dipeptide hydrolase with a calculated molecular mass of 120-150 kDa. ACE is widely detected in the endothelial cell of organ tissues included lung, brain, kidney. ACE catalyzes the formation of angiotensin I to angiotensin II, which could cause vasoconstriction, promote synthesis and release of adrenocortical hormone aldosterone. It is important to detect ACE activity for diagnosis and treatment of lung, liver, thyroid and other organ diseases.

Measurement Principle：

ACE could catalyze FAPGG to form FAP and glycylglycine. FAPGG has a characteristic absorption peak at 340 nm, and ACE activity is calculated by measuring the rate of decrease in absorbance at 340nm.

Self Provided：

Spectrophotometer/Microplate reader, desk centrifuge, balance, transferpettor, constant temperature foster box/water-bath, micro quartz cuvette/96 well UV plate, mortar/homogenizer/cell ultrasonic crusher, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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