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Application： ELISA
Reactivity：Common Species
Sensitivity：0.94 ng/ml
Storage：2-8℃，6 months
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| Name | HIS ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | HIS |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Common Species |
| Application | ELISA |
| Reaction Type | Competitive-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 1.563-100 ng/ml |
| Sensitivity | 0.94 ng/ml |
| Specificity | Can detect HIS in the sample, and there is no significant crossover with other related proteins. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 1.25h |
| Detection Principle | Solarbio ELISA试剂盒采用基于竞争法的酶联免疫吸附检测技术。将HIS抗原包被于酶标板上；实验时，分别加入梯度稀释的标准品和预稀释的样本，然后加入生物素标记的抗HIS单抗，样本或标准品中的HIS与包被的HIS竞争生物素标记的抗HIS单抗上的结合位点；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样本存在不同浓度的HIS，则HRP会使无色TMB变成不同深浅（负相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），HIS浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中HIS的浓度。 |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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