Cerebrospinal Fluid Total Protein Test Kit (Dye Binding colorimetry)

Product introduction

Cerebro-Spinal Fluid (CSF) is a colorless transparent fluid that exists in the cerebral ventricle, subarachnoid space and central canal of the spinal cord. It is produced by the choroid plexus in the cerebral ventricle. It has similar properties to plasma and lymph. Normal cerebrospinal fluid has a certain chemical composition and pressure, which plays an important role in maintaining the relative stability of intracranial pressure. When the central nervous system is damaged, the detection of cerebrospinal fluid becomes an important auxiliary diagnostic method. Total Protein (TP) is composed of albumin and globulin. The determination of TP in biological fluids (serum, urine, cerebrospinal fluid) is generally based on the following two assumptions: 1. All protein molecules are composed of pure peptides with a mass percentage of nitrogen content of 16%; 2. There are hundreds of protein molecules in body fluids, and each molecule has very similar characteristics to the determination reaction. At present, the commonly used methods for the detection of total protein are: biuret method, ultraviolet spectrophotometry, dye binding method, Kjeldberg nitrogen method, precipitation method, etc.

The detection principle of the kit is that under acidic conditions, eosin dissociates into negative ion type, and the dye matgrand color gradually fades, so that the blank absorbance of the reagent decreases. Arginine, histidine, lysine and tryptophan residues in protein peptides were dissociated into -NH3+ groups, which were combined with eosin to form red protein complex. The absorbance of the complex was proportional to the protein concentration. Compared with the standard liquid treated with the same method, the content of protein in the sample was measured, which could be used for the determination of total protein content in human or animal cerebrospinal fluid samples.

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Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.