Cerebrospinal fluid Total protein Detection Kit (Turbidity microplate method)

Product introduction

Cerebro-Spinal Fluid (CSF) is a colorless transparent fluid that exists in the cerebral ventricle, subarachnoid space and central canal of the spinal cord. It is produced by the choroid plexus in the cerebral ventricle. It has similar properties to plasma and lymph. Normal cerebrospinal fluid has a certain chemical composition and pressure, which plays an important role in maintaining the relative stability of intracranial pressure. When the central nervous system is damaged, the detection of cerebrospinal fluid becomes an important auxiliary diagnostic method. Total Protein (TP) is composed of albumin and globulin. The determination of TP in biological fluids (serum, urine, cerebrospinal fluid) is generally based on the following two assumptions: 1. All protein molecules are composed of pure peptides with a mass percentage of nitrogen content of 16%; 2. There are hundreds of protein molecules in body fluids, and each molecule has very similar characteristics to the determination reaction. At present, the commonly used methods for the detection of total protein are: biuret method, ultraviolet spectrophotometry, dye binding method, Kjeldberg nitrogen method, precipitation method, etc.

The detection principle of this kit is that the protein in cerebrospinal fluid and sulfosalicylic acid interact to form precipitation, and the turbidity is measured by turbidimetry. Compared with the standard solution with the same treatment, the content of protein in the sample is calculated. It can be used for the determination of total protein content in human or animal cerebrospinal fluid samples, but it is easily affected by surfactant.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.