HCT-116 Whole Cell RIPA Extract
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Cat.No：SCC-110714-L10 Solarbio
Storage：Store at -20℃. Avoid freeze/thaw cycles.
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PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | HCT-116 Whole Cell RIPA Extract |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Concentration | 2.5mg/ml |
| Unit | Piece |
| Specification | 100ug 500ug |
产品概述
HCT-116 cells were lysed using radio immunoprecipitation assay (RIPA) buffer supplemented with protease and phosphatase inhibitors, centrifuged and the supernatant collected.100 μg of cellular protein was mixed with 4 times volume of 5X SDS-PAGE loading buffer(with DTT).
应用
WB;SDS-PAGE
产品类型
SDS-PAGE Protein Sample;Cell Lysate
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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