L-Rhamnose Monohydrate
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Cat.No：SR8080 Solarbio
CAS：6155-35-7
Purity：HPLC≥98%
Appearance：White to off-white powder
Storage：Store at RT，2 years.
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Biotin-Conjugated
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Allophycocyanin(APC)-Conjugated
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Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
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Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| CAS | 6155-35-7 |
| Name | L-Rhamnose Monohydrate |
| Purity | HPLC≥98% |
| Molecular Formula | C6H12O5·H2O |
| Molecular Weight | 182.17 |
| Appearance | White to off-white powder |
| Solubility | ≥5mg/mL in Water |
| Storage | Store at RT，2 years. |
| EC | EINECS 222-793-4 |
| MDL | MFCD00071591 |
| SMILES | O[C@@H]([C@@H](O[C@H]1O)C)[C@H]([C@H]1O)O.O |
| InChI | InChI=1S/C6H12O5.H2O/c1-2-3(7)4(8)5(9)6(10)11-2;/h2-10H，1H3;1H2/t2-，3-，4+，5+，6+;/m0./s1 |
| Note | The reserve liquid is recommended to be packaged and frozen to avoid repeated freezing and thawing (-20℃, 1 month); The diluted working liquid is recommended to be used and prepared immediately |
| Unit | Bottle |
| Specification | 20mg |
Examples of using this product（for reference only）
HPGPC
CSPs (5.0 mg) was dissolved in 1.0 mL pure water and injected into the Shodex OH pak SB-804 column (300 mm × 8.0 mm i.d.). The flow rate of the mobile phase was 0.8 mL/min. The injection volume was 20 μL. T-series dextran was used as a standard.
References：
Jia Y, Xue Z, Wang Y, Lu Y, Li R, Li N, Wang Q, Zhang M, Chen H. Chemical structure and inhibition on α-glucosidase of polysaccharides from corn silk by fractional precipitation. Carbohydr Polym. 2021 Jan 15;252:117185. doi: 10.1016/j.carbpol.2020.117185. Epub 2020 Oct 2. PMID: 33183632.
IC
Ion chromatography was employed to determine the monosaccharide composition of the PCP-100, and it was coupled to a column (150 mm × 3 mm) and a pulsed amperometric detector.The PCP-100 (1 mg) was hydrolyzed by adding trifluoroacetic acid (2 M, 1 mL) for 3 h at 110 ℃. Following drying under nitrogen, the hydrolysate was mixed thoroughly with methanol to completely remove the excess trifluoroacetic acid. Subsequently, the hydrolysate was diluted with ultrapure water, and the injection volume was 1 mL. The mobile phase consisted of two solvents, in which mobile phase A was sodium hydroxide and mobile phase B was sodium acetate in sodium hydroxide. The flow rate was set at 0.4 mL/min and the column temperature was set at 30 ℃.
References：
Chen Y, Song L, Chen P, Liu H, Zhang X. Extraction, Rheological, and Physicochemical Properties of Water-Soluble Polysaccharides with Antioxidant Capacity from Penthorum chinense Pursh. Foods. 2023 Jun 10;12(12):2335. doi: 10.3390/foods12122335. PMID: 37372546; PMCID: PMC10297149.
GC
The six tea polysaccharides were hydrolyzed in 2 mol/L trifluoroacetic acid at 100 ℃ for 6 hr, respectively . The hydrolysate was reduced by NaBH4 and acetylation with acetic anhydride, converting into its respective alditol acetate. The derivative was analyzed by a GC (GC-7900, Shanghai, China) with a capillary column (HP-5, 30.0 m × 0.32 nm × 0.5 mm). The temperatures of injector and detector were 250 and 280 ℃, respectively . N2 was used as the carrier gas and maintained at 1.0 mL/min.
References：
Xu L, Chen Y, Chen Z, Gao X, Wang C, Panichayupakaranant P, Chen H. Ultrafiltration isolation, physicochemical characterization, and antidiabetic activities analysis of polysaccharides from green tea, oolong tea, and black tea. J Food Sci. 2020 Nov;85(11):4025-4032. doi: 10.1111/1750-3841.15485. Epub 2020 Oct 10. PMID: 33037621.
HPLC
HPLC analysis was performed on an LC20A HPLC system (Shimadzu, Japan) equipped with an SPD-20A ultraviolet detector and a C18 column (250 mm×4.6 mm, 5 μm, Shimadzu, Japan). The mobile phase was a mixture of 0.1 mol/L NaH2PO4-Na2HPO4 buffer (pH 6.7) and acetonitrile (83:17), and a flow rate of 1.0 mL/min was used. The wavelength of detection was 245 nm, and the column temperature was 30?C.
References：
Chen X, Sheng Z, Qiu S, Yang H, Jia J, Wang J, Jiang C. Purification, characterization and in vitro and in vivo immune enhancement of polysaccharides from mulberry leaves. PLoS One. 2019 Jan 2;14(1):e0208611. doi: 10.1371/journal.pone.0208611. PMID: 30601811; PMCID: PMC6314569.
TPS-MFGDP-MS
The conditions of mass spectrometer were optimized as follows: capillary voltage, 18 V; capillary temperature, 320 ℃; tube lens, 20 V. All spectra were collected under automatic gain mode where per spectrum was set 3 microscans at 100 ms maximum ion injection time. The flow of He through the channel was controlled by a mass flow controller (D08-1F3L, Sevenstar, Beijing, China) at a rate of 1 mL min ?1.
References：
Li Y, Guo X, Tian Y, Zhang T, Luo Z, Liu X, Qian C, Dai J, Duan Y. Methylation in combination with temperature programming enables rapid identification of polysaccharides by ambient micro-fabrication glow discharge plasma (MFGDP) desorption ionization mass spectrometry. Talanta. 2020 Oct 1;218:121156. doi: 10.1016/j.talanta.2020.121156. Epub 2020 May 15. PMID: 32797915.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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